LymphedemaV1, Main, Exploration, bibRecord, 004780

Generation of conditional alleles for Foxc1 and Foxc2 in mice

Identifieur interne : 004780 ( Main/Exploration ); précédent : 004779; suivant : 004781

Generation of conditional alleles for Foxc1 and Foxc2 in mice

Auteurs : Amy Sasman [États-Unis] ; Carey Nassano-Miller [États-Unis] ; Kyoo Seok Shim [États-Unis] ; Hyun Young Koo [États-Unis] ; Ting Liu [États-Unis] ; Kathryn M. Schultz [États-Unis] ; Meredith Millay [États-Unis] ; Atsushi Nanano [États-Unis] ; Myengmo Kang [États-Unis] ; Takashi Suzuki [États-Unis] ; Tsutomu Kume [États-Unis]

Source :

genesis [ 1526-954X ] ; 2012-10.

RBID : ISTEX:814AB4C97D2BAA7D95908A7B7F70742F780C0185

Abstract

The Forkhead box transcription factors, Foxc1 and Foxc2, are crucial for development of the eye, cardiovascular network, and other physiological systems, but their cell‐type specific and postdevelopmental functions are unknown, in part because conventional (i.e., whole‐organism) homozygous‐null mutations of either factor result in perinatal death. Here, we describe the generation of mice with conditional‐null Foxc1flox and Foxc2flox mutations that are induced via Cre‐mediated recombination. Mice homozygous for the unrecombined alleles are viable and fertile, indicating that the conditional alleles retain their wild‐type function. The embryos of Foxc1flox or Foxc2flox mice crossed with Cre‐deleter mice that are homozygous for the recombined allele (i.e., Foxc1Δ/Δ or Foxc2Δ/Δ embryos) lack expression of the corresponding gene and show the same developmental defects observed in conventional homozygous mutant embryos. We expect these conditional mutations to enable characterization of the cell‐type specific functions of Foxc1 and Foxc2 in development, disease, and adult animals. genesis 50:766–774, 2012. © 2012 Wiley Periodicals, Inc.

Url:

DOI: 10.1002/dvg.22036

Affiliations:

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Generation of conditional alleles for Foxc1 and Foxc2 in mice</title>
<author><name sortKey="Sasman, Amy" sort="Sasman, Amy" uniqKey="Sasman A" first="Amy" last="Sasman">Amy Sasman</name>
</author>
<author><name sortKey="Nassano Iller, Carey" sort="Nassano Iller, Carey" uniqKey="Nassano Iller C" first="Carey" last="Nassano-Miller">Carey Nassano-Miller</name>
</author>
<author><name sortKey="Shim, Kyoo Seok" sort="Shim, Kyoo Seok" uniqKey="Shim K" first="Kyoo Seok" last="Shim">Kyoo Seok Shim</name>
</author>
<author><name sortKey="Koo, Hyun Young" sort="Koo, Hyun Young" uniqKey="Koo H" first="Hyun Young" last="Koo">Hyun Young Koo</name>
</author>
<author><name sortKey="Liu, Ting" sort="Liu, Ting" uniqKey="Liu T" first="Ting" last="Liu">Ting Liu</name>
</author>
<author><name sortKey="Schultz, Kathryn M" sort="Schultz, Kathryn M" uniqKey="Schultz K" first="Kathryn M." last="Schultz">Kathryn M. Schultz</name>
</author>
<author><name sortKey="Millay, Meredith" sort="Millay, Meredith" uniqKey="Millay M" first="Meredith" last="Millay">Meredith Millay</name>
</author>
<author><name sortKey="Nanano, Atsushi" sort="Nanano, Atsushi" uniqKey="Nanano A" first="Atsushi" last="Nanano">Atsushi Nanano</name>
</author>
<author><name sortKey="Kang, Myengmo" sort="Kang, Myengmo" uniqKey="Kang M" first="Myengmo" last="Kang">Myengmo Kang</name>
</author>
<author><name sortKey="Suzuki, Takashi" sort="Suzuki, Takashi" uniqKey="Suzuki T" first="Takashi" last="Suzuki">Takashi Suzuki</name>
</author>
<author><name sortKey="Kume, Tsutomu" sort="Kume, Tsutomu" uniqKey="Kume T" first="Tsutomu" last="Kume">Tsutomu Kume</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:814AB4C97D2BAA7D95908A7B7F70742F780C0185</idno>
<date when="2012" year="2012">2012</date>
<idno type="doi">10.1002/dvg.22036</idno>
<idno type="url">https://api.istex.fr/document/814AB4C97D2BAA7D95908A7B7F70742F780C0185/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">003C96</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">003C96</idno>
<idno type="wicri:Area/Istex/Curation">003C96</idno>
<idno type="wicri:Area/Istex/Checkpoint">000477</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000477</idno>
<idno type="wicri:doubleKey">1526-954X:2012:Sasman A:generation:of:conditional</idno>
<idno type="wicri:source">PMC</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435482</idno>
<idno type="RBID">PMC:3435482</idno>
<idno type="wicri:Area/Pmc/Corpus">003440</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003440</idno>
<idno type="wicri:Area/Pmc/Curation">003439</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003439</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002992</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002992</idno>
<idno type="wicri:Area/Ncbi/Merge">004C98</idno>
<idno type="wicri:Area/Ncbi/Curation">004C98</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">004C98</idno>
<idno type="wicri:doubleKey">1526-954X:2012:Sasman A:generation:of:conditional</idno>
<idno type="wicri:Area/Main/Merge">004805</idno>
<idno type="wicri:Area/Main/Curation">004780</idno>
<idno type="wicri:Area/Main/Exploration">004780</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Generation of conditional alleles for <hi rend="italic">Foxc1</hi>
 and <hi rend="italic">Foxc2</hi>
 in mice</title>
<author><name sortKey="Sasman, Amy" sort="Sasman, Amy" uniqKey="Sasman A" first="Amy" last="Sasman">Amy Sasman</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Illinois</region>
</placeName>
<wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Nassano Iller, Carey" sort="Nassano Iller, Carey" uniqKey="Nassano Iller C" first="Carey" last="Nassano-Miller">Carey Nassano-Miller</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Illinois</region>
</placeName>
<wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Shim, Kyoo Seok" sort="Shim, Kyoo Seok" uniqKey="Shim K" first="Kyoo Seok" last="Shim">Kyoo Seok Shim</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Illinois</region>
</placeName>
<wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Koo, Hyun Young" sort="Koo, Hyun Young" uniqKey="Koo H" first="Hyun Young" last="Koo">Hyun Young Koo</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Illinois</region>
</placeName>
<wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Liu, Ting" sort="Liu, Ting" uniqKey="Liu T" first="Ting" last="Liu">Ting Liu</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Illinois</region>
</placeName>
<wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Schultz, Kathryn M" sort="Schultz, Kathryn M" uniqKey="Schultz K" first="Kathryn M." last="Schultz">Kathryn M. Schultz</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Illinois</region>
</placeName>
<wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Millay, Meredith" sort="Millay, Meredith" uniqKey="Millay M" first="Meredith" last="Millay">Meredith Millay</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Illinois</region>
</placeName>
<wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Nanano, Atsushi" sort="Nanano, Atsushi" uniqKey="Nanano A" first="Atsushi" last="Nanano">Atsushi Nanano</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>Department of Medicine, Vanderbilt University Medical Center, Nashville</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Kang, Myengmo" sort="Kang, Myengmo" uniqKey="Kang M" first="Myengmo" last="Kang">Myengmo Kang</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>Department of Medicine, Vanderbilt University Medical Center, Nashville</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Suzuki, Takashi" sort="Suzuki, Takashi" uniqKey="Suzuki T" first="Takashi" last="Suzuki">Takashi Suzuki</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>Department of Medicine, Vanderbilt University Medical Center, Nashville</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Kume, Tsutomu" sort="Kume, Tsutomu" uniqKey="Kume T" first="Tsutomu" last="Kume">Tsutomu Kume</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Illinois</region>
</placeName>
<wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea>
</affiliation>
<affiliation></affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">genesis</title>
<title level="j" type="alt">GENESIS</title>
<idno type="ISSN">1526-954X</idno>
<idno type="eISSN">1526-968X</idno>
<imprint><biblScope unit="vol">50</biblScope>
<biblScope unit="issue">10</biblScope>
<biblScope unit="page" from="766">766</biblScope>
<biblScope unit="page" to="774">774</biblScope>
<biblScope unit="page-count">9</biblScope>
<publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="2012-10">2012-10</date>
</imprint>
<idno type="ISSN">1526-954X</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1526-954X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The Forkhead box transcription factors, Foxc1 and Foxc2, are crucial for development of the eye, cardiovascular network, and other physiological systems, but their cell‐type specific and postdevelopmental functions are unknown, in part because conventional (i.e., whole‐organism) homozygous‐null mutations of either factor result in perinatal death. Here, we describe the generation of mice with conditional‐null Foxc1flox and Foxc2flox mutations that are induced via Cre‐mediated recombination. Mice homozygous for the unrecombined alleles are viable and fertile, indicating that the conditional alleles retain their wild‐type function. The embryos of Foxc1flox or Foxc2flox mice crossed with Cre‐deleter mice that are homozygous for the recombined allele (i.e., Foxc1Δ/Δ or Foxc2Δ/Δ embryos) lack expression of the corresponding gene and show the same developmental defects observed in conventional homozygous mutant embryos. We expect these conditional mutations to enable characterization of the cell‐type specific functions of Foxc1 and Foxc2 in development, disease, and adult animals. genesis 50:766–774, 2012. © 2012 Wiley Periodicals, Inc.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Illinois</li>
<li>Tennessee</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Illinois"><name sortKey="Sasman, Amy" sort="Sasman, Amy" uniqKey="Sasman A" first="Amy" last="Sasman">Amy Sasman</name>
</region>
<name sortKey="Kang, Myengmo" sort="Kang, Myengmo" uniqKey="Kang M" first="Myengmo" last="Kang">Myengmo Kang</name>
<name sortKey="Koo, Hyun Young" sort="Koo, Hyun Young" uniqKey="Koo H" first="Hyun Young" last="Koo">Hyun Young Koo</name>
<name sortKey="Kume, Tsutomu" sort="Kume, Tsutomu" uniqKey="Kume T" first="Tsutomu" last="Kume">Tsutomu Kume</name>
<name sortKey="Liu, Ting" sort="Liu, Ting" uniqKey="Liu T" first="Ting" last="Liu">Ting Liu</name>
<name sortKey="Millay, Meredith" sort="Millay, Meredith" uniqKey="Millay M" first="Meredith" last="Millay">Meredith Millay</name>
<name sortKey="Nanano, Atsushi" sort="Nanano, Atsushi" uniqKey="Nanano A" first="Atsushi" last="Nanano">Atsushi Nanano</name>
<name sortKey="Nassano Iller, Carey" sort="Nassano Iller, Carey" uniqKey="Nassano Iller C" first="Carey" last="Nassano-Miller">Carey Nassano-Miller</name>
<name sortKey="Schultz, Kathryn M" sort="Schultz, Kathryn M" uniqKey="Schultz K" first="Kathryn M." last="Schultz">Kathryn M. Schultz</name>
<name sortKey="Shim, Kyoo Seok" sort="Shim, Kyoo Seok" uniqKey="Shim K" first="Kyoo Seok" last="Shim">Kyoo Seok Shim</name>
<name sortKey="Suzuki, Takashi" sort="Suzuki, Takashi" uniqKey="Suzuki T" first="Takashi" last="Suzuki">Takashi Suzuki</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004780 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004780 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:814AB4C97D2BAA7D95908A7B7F70742F780C0185
   |texte=   Generation of conditional alleles for Foxc1 and Foxc2 in mice
}}

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

Serveur d'exploration sur le lymphœdème

Generation of conditional alleles for Foxc1 and Foxc2 in mice

Generation of conditional alleles for Foxc1 and Foxc2 in mice

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.